TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
ISSN: 2525 - 2372
TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
Nghiên cứu được thực hiện để xác định thành phần hóa học của tinh dầu tiêu đen chiết xuất từ hạt hồ tiêu đen tại tỉnh Đăk Nông, Việt Nam. Phổ GC-MS ghi nhận trong tinh dầu tiêu chứa 13 hợp chất hữu cơ với thành phần chính là 3-Carane (25,6%), b-Caryophyllene (20,7%), Limonene (21,6%). Đánh giá khả năng kháng khuẩn của tinh dầu tiêu trên 5 chủng vi khuẩn E. coli, B. cereus, P. pseumollei, Salmonella, và S. aureus ở các nồng độ từ 4,4 - 88 mg/mL thì kết quả đường kính vòng tròn vô khuẩn tăng dần và đạt giá trị cực đại tại nồng độ 88 mg/mL lần lượt là 18,38; 15,88; 13,88; 15,25; 12,25 mm, so với đường kính vô khuẩn của kháng sinh Amoxicilin 10 mg/mL là 6,63; 18,38; 10,00; 19,25; 6,38 mm thì khả năng kháng khuẩn của tinh dầu tiêu đen có thể ngang bằng hay thậm chí còn tốt hơn so với dung dịch Amoxicilin. Nồng ức chế tối thiểu MIC của tinh dầu tiêu đen trên 5 chủng vi khuẩn E. coli, B. cereus, P. pseumollei, Salmonella, và S. aureus lần lượt là 128, 2048, 1024, 256 và 2048 µg/mL.
The study was conducted to identify the chemical compound of black pepper essential oil extracted from black pepper seed cultivated in Dak Nong province, Vietnam. According to GC-MS analysis, black pepper essential oil contains 13 chemical compounds, the majority of which are 3-Carane (25,6%), b-Caryophyllene (20,7%), Limonene (21,6%), all of which are biologically-active compounds. The antibacterial activity of pepper essential oil on 5 pathogenic bacteria strains E. coli, B. cereus, P. pseumollei, Salmonella, and S. aureus from 4,4 to 88 mg/mL was evaluated. The results show that, at the concentrations from 4,4 to 88mg/ml, the diameter of the antibacterial circle increases steadily and reaches the peaks at the concentration of 88 mg/mL, which is 18,38; 15,88; 13,88; 15,25; 12,25 mm respectively, compared to the diameter of the antibacterial circle of Amoxicillin 10 mg/mL is 6,63; 18,38; 10,00; 19,25; 6,38 mm, showing that the antibacterial activity of black pepper essential oil can be equal to or even better than that of the antibiotic Amoxicillin 10 mg/mL under the same analysis condition. The minimum inhibitory concentration (MIC) values of black pepper essential oil on 5 strains of E. coli, B. cereus, P. pseumollei, Salmonella, and S. aureus are 128, 2048, 1024, 256, and 2048 µg/mL respectively.
tinh dầu tiêu đen, hoạt chất sinh học, vi khuẩn gây hư hỏng thực phẩm, hoạt tính kháng khuẩn, MIC.
Black pepper essential oil, biologically-active compounds, spoilage bacteria, antibacterial activity, MIC.
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